You should be fine, just restore back to stock OS 3.0 & re-jailbreak with Redsn0w.
I've used Redsn0w with Vista & Windows 7. Make sure screensavers are turned off & stuff like that.
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White iPhone 3GS 32GB
Firmware 3.1.2 jailbroken with Pwnagetool 3.1.4 on my PC running OS X Leopard & Windows 7 Ultimate!
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